Real time PCR assay for detection of bacterial respiratory pathogens

摘要

Methods for detecting Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella spp. are disclosed. A sample suspected of containing a nucleic acid of one or more of M. pneumoniae, C. pneumoniae, and Legionella spp. is screened for the presence or absence of that nucleic acid. Determining whether the M. pneumoniae, C. pneumoniae, or Legionella spp. nucleic acid is present in the sample can be accomplished by detecting hybridization between a M. pneumoniae probe (such as a CARDS toxin probe), a C. pneumoniae probe (such as a ArgR probe), or a Legionella spp. probe (such as a SsrA probe) and a nucleic acid in a sample. Also disclosed are probes and primers for the detection of M. pneumoniae, C. pneumoniae, and Legionella spp., and kits that contain the disclosed probes and/or primers.