Methods and compositions for high efficiency transfection of siRNA

摘要

Described herein are methods and compositions for high efficiency transfection of siRNA into a cell population. Such methods and compositions utilize a low voltage pre-conditioning pulse to modulate the efficiency of siRNA transfection. In some embodiments, the methods and compositions permit spatial and temporal control of siRNA transfection efficiency within a population of cells. The disclosed methods and compositions, in some embodiments, are amenable to high throughput applications such as siRNA library-based phenotypic screening.