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Fluorescence energy transfer by competitive hybridization
Fluorescence energy transfer by competitive hybridization
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机译:竞争性杂交的荧光能量转移
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摘要
A method is provided for detecting the presence of nucleotides or monitoring nucleotide amplification. It utilizes fluorescence energy transfer by competitive hybridization. Competitive hybridization is achieved by using unequal length complementary probes which have a fluorophore on one probe and a quencher on the other. The fluorophore and quencher are juxtaposed in a manner wherein the proximity of the quencher to the fluorophore produces quenching of the fluorescence of the fluorophore.
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