recombinant trypanosoma cruzi and vaccine composition for leishmaniasis prophylaxis

摘要

trypanosoma cross! Recombinant and Leishmaniasis Prophylaxis Vaccine Composition The present invention describes the non-virulent trypanosoma cruzi strain cl-14 transfected with an integrative vector containing an optimized synthetic leishmania a2 protein gene (seq id no01) as well as a vaccine composition which a contains and uses, of t. recombinant cruzi and vaccine composition. immunization with the cl-14 4 a2 construct potentiated cell response to a2 antigen and derived peptides, associated with high ifn-y t 0d4 + and cd8 + cells, and was able to induce partial protection in balb / c mice, against infection by l. infantum. Therefore, the strategy used represents an approach capable of enhancing immunogenicity and protection against visceral ieishmaniasis, being an alternative strategy for the development of vaccine compositions. In addition, the cl-14 a2 construct enables the production of recombinant protein in eukaryotic cells for diagnostic testing.