Simultaneous crosslinking process, sterilization and production of polymeric system containing protein nanoparticles

摘要

The application of invention patent "process simultaneously cross-linking, sterilization and production of polymeric system containing protein nanoparticles.The present invention relates to the development of a polymeric system containing nano particles of protein occurring in a single step synthesis of protein cross-linking, the cross-linking of the polymer and the sterilization of the final product, and the induced protein cross-linking Occurs within the polymeric matrix (in situ), all at the same time, promoted by exposure to ionizing radiation.The said process uses small amounts of polymer and protein, provides more stability to the protein used, reduces the degradation of protein assigned to each step eliminated, as well as resulting in a final product which consists of a film or hydrogel containing protein, whose pa Rtu00edculas have medium size controlled, preserved bioactivity, good stability and biocompatibility.In addition to other specific technical characteristics. Surprisingly, the job control of radiation associated with the experimental conditions adopted, made it possible to combine the crosslinking of protein and polymer present in the solution, leading to the formation of the said system.It is interesting to note that the employment of radiation, in this case, not only allows the decrease of any steps associated with the production of nanoparticulados systems, such as sterilization and ensures the synthesis of nanoparticles simultaneously.In this way, the process is simplified, more rapid and enables the realization of the procedure on a large scale, without the generation of toxic waste, or the use of monomers. The final product obtained can be used as a system to use or shipment of drugs or drugs of low toxicity or biomedical device with bioactivity.