Modification method of surfaces with proteins for preconcentration of analyte for desorption-ionization techniques of mass spectrometry and immunochemical assays

摘要

ufeffpodstatou technical solution is the way in which the modification of solid substrates for the active surface prekoncentraci protein analyte of compound samples before detection based on desorpu010dnu011b - ionization mass spectrometry and imunochemicku00fdch essays.the subject of protection is a method of modification of surfaces used as substrates for desorpu010dnu011b - ionization mass spectrometry and immunochemical essays. the way is based on the elektronebulizaci (elektrosprejovu00e1nu00ed) solution of proteins, depending on the intended application lektinu016f enzymes or antibodies.the loaded elektrosprej is dried in real time through odpau0159ovacu00edm space and formed by a desolvatovanu00fdch ions affects the surface to which is firmly established.this modified surface can then be used for the selective interaction with partner proteins afinitnu00edm deponovanu00e9, his prekoncentracu00ed or enzymatic modification with subsequent analysis desorpu010dnu011b - ionization mass spectrometry or by imunochemicku00fdch essays.