Detection method of gene expression of molecular markers characterizing adipogenic differentiation of stem cells specific for animal model of a horse, panel of primers for making the method and use of such a primer panel

摘要

Panel of primers and method of detection of gene expression of molecular markers characterising adipogenic differentiation specific to the animal model of the horse,where a polymerase chain reaction (PCR) is carried out using a specimen panel consisting of:oligonucleotide pair: PPAR alfa F: 5 -GGA AGT GGT CCA GGA TCA GA -3 (20 -mer), PPAR alfa R: 5 -AGG CAT GAA CTC CGT AAT GG -3 (20 -mer),for the detection of the alpha gene sequence of the PPAR receptor and subsequently electrophoresis of the PCR reaction products shall be performed.The invention solution allows the rapid, simple and specific detection of cells differentiated into adipocytes by means of a specific marker in cell isolates especially in the animal species of the horse.