A PROCESS FOR PREPARING DNA CODING FOR SHIGELLA ATPASE ACTIVITY

摘要

A PROCESS FOR PREPARING DNA CODING FOR SHIGELLA ATPase ACTIVITY. ABSTRACT The Invention is directed to a process for preparing plasmid DNA coding for Shigella ATPase activity comprises cutting megaplasmld DNA from Shigella taken by known methods into fragments by a restriction enzyme Hind III such as herein described: cutting into fragments commercially available plasmld (DNA) PUC8 by the restriction enzyme, Hind III mixing the fragments obtained from both the DNAs adding enzyme T4 DNA llgase to such mixture, Transforming by known methods the ligat.ed DNA into E.Coli, selecting by known testing methods from it the E.Coli strains carrying desired recombinants, e.g. those which are resistant to ampicillin, recombinant colonies or pools of such colonies so selected being tested for ATPase activity and based on this test selecting specific E.Coli recombinant carrying the Shigella ATPase activity and isolating by known methods plasmld DNA encoding Shigella ATPase activity, whereafter, if desired the DNA sequence is subjected to hybridization in a known manner to produce corresponding hybridization probe.