A PROCESS FOR ISOLATION AND CHARACTERIZATION OF ANGIOTENSIN CONVERTING ENZYME

摘要

The present invention provides the process for the isolation of an active component which is a glycoside from Moringa oleifera (Lam.) root useful in the treatment of epilepsy which comprises preparing an methanol extract from Moringa oleifera (Lam.) root ,column chromatography and Thin layer column chromatography for the separation of active components. Fourier transforms infrared (FTIR) spectroscopy for analysis of functional groups in the active component; identification of proton and carbon of the active component by Nuclear Magnetic Resonance (NMR) spectroscopy. Two dimensional nuclear magnetic resonance (2D NMR) spectroscopy for resolving and identifying the nature of chemical sites in the sample; HSQC (Heteronuclear Single Quantum Coherence) for the detection of bond couplings; lH - H COSY (Homonuclear Correlation Spectroscopy) for revealing correlations between coupled protons; HMBC (Heteronuclear Multiple Bond Coherence) also for the detection of bond couplings; Mass Spectrometer for the molecular weight determination the overall analysis indicating a structure of the active component of the said extract with a Molecular Formula = C39H62O26; Molecular weight: 969.0 grams/mole and the Compound name: 3-phenylpropanol-pentaglycosides. In addition, the active component was found to exhibit receptor binding affinity towards GABA(A) a-1 receptor, which apart from being novel and hitherto not known, provide a highly efficient, convenient, safe and cheap method for the treatment of epilepsy.