首页> 外国专利> REAGENT ALLOWING FOR MICROORGANISM INACTIVATION EXTRACTION AND STORAGE OF BACTERIAL DNA IN FORM SUITABLE FOR HIGHLY EFFECTIVE MOLECULAR DIAGNOSTICS

REAGENT ALLOWING FOR MICROORGANISM INACTIVATION EXTRACTION AND STORAGE OF BACTERIAL DNA IN FORM SUITABLE FOR HIGHLY EFFECTIVE MOLECULAR DIAGNOSTICS

机译:微生物活化提取和细菌DNA储存所需的试剂,其形式适用于高效分子诊断

摘要

The invention relates to biochemistry, chemistry and medicine field. A reagent for the treatment and storage of a biological sample intended for in vitro diagnostics of infectious disease via PCR method, said reagent being an aqueous solution containing an alcohol, an alkali and a tertiary amine in concentrations providing for amplification efficiency increase for at least 2 cycles, is described. The said reagent provides for neutralization of products contained in biological sample and inhibiting PCR reaction, microorganism inactivation, increase of biological sample shelf life and DNA isolation efficiency.
机译:本发明涉及生物化学,化学和医学领域。一种旨在通过PCR方法进行感染性疾病体外诊断的生物样品的处理和储存试剂,所述试剂是含有酒精,碱和叔胺的水溶液,其浓度可使扩增效率至少提高2倍循环,描述。所述试剂用于中和生物样品中包含的产物,并抑制PCR反应,微生物失活,增加生物样品的保存期限和DNA分离效率。

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