Bis1-phenyl-3-methyl-6-(pyridinium-4-il)-4,5-diaza-hexa-1,3,5-triene-1-hydroxy-6-olato-O1,N4,O6iron(II)sulphate tetrahydrate and process for cultivation of microalga Porphyridium cruentum with its use

摘要

The invention relates to chemistry and biotechnology, in particular to the synthesis of a new coordinative compound of iron(II) which can be used to produce a biomass with a high antioxidant capacity and which can be used in the food industry and in medicine, and to a process for cultivation of microalga Porphyridium cruentum with its use.According to the invention, a coordinative compound - βis[1-phenyl-3-methyl-6-(pyridinium-4-il)-4,5-diaza-hexa-1,3,5-triene-1-hydroxy-6-olato-O1,N4,O6]iron(II)sulphate tetrahydrate is claimed.Also, claimed is a process for cultivation of microalga Porphyridium cruentum, which consists in that microalga is cultivated on a nutrient medium containing, g/L: NaNO3 - 5.0; NaCl - 7.0; KCl - 7.5; MgSO4·7H2O - 1.8; Ca(NO3)2·4H2O - 0.15; KBr - 0.05; KI - 0.05; K2HPO4 - 0.2; ZnSO4·5H2O - 0.00002; CuSO4·5H2O - 0.00005; MnSO4·5H2O - 0.0003; H3BO3 - 0.0006; MoO3 - 0.00002; NaVO3 - 0.00005, the compound βis[1-phenyl-3-methyl-6-(pyridinium-4-il)-4,5-diaza-hexa-1,3,5-triene-1-hydroxy-6-olato-O1,N4,O6]iron(II)sulphate tetrahydrate - 0.011…0.012 and distilled water up to 1 L; having the pH 6.8…7.2, at the temperature of 23…25°C, the illumination of 2000…3000 lx/cm2, with periodic slow agitation.The result consists in increasing the phenol content in the microalga biomass.