ELISA substitute kit for detection of anti flash bacterial psychrophilum antibody in rainbow trout

摘要

This is a substitute ELISA package for the detection of anti flash memory bacteria psycorphilum antibody in rainbow trout, which includes: (a) pocket loaded microplate; (b) trout serum containing psycorphilum NCIB 1947, the main anti flash memory bacteria; and (c) rainbow trout serum without the main antibody; (d) the secondary antibodies of mice to the main anti flavocheterium psychrophhilum NCIB antibodies of trout in 1947, which are used as enzyme markers to bind with peroxides (HRP);And (E) a solution containing tetramethylbenzodiazepine (TMB) as a substrate for enzyme HRP operation. It also refers to the method for detecting the antibody against butter bacteria and the currently invented ELISA kit; the method for separating the main antibody from the rainbow trout serum; and the method for extracting the secondary antibody from the B / c serum of mice and its copolymers. And HRP