Method for producing polymer with the promoter sequence TATAAA in artificial cassette TATA and application of this polymer for recognizing and/or selectively determining hexanucleotide sequence of DNA.

摘要

The object of the invention is to obtain a layer of polymer that recognizes amino acids selected from the amino acids present in the binding protein TATA, attached by the TATA tape, by a molecular embodiment method leading to the production of a molecular embossed polymer (MIP),by electrochemical polymerisation under potential dynamic conditions. In this implementation, 4-[2-(6-amino-9H-purin-9-yl)ethoxy]phenyl-4-[bis(2,2'-bitien-5-yl)methane] and 4-(di-2,2'-bitioacetate-5-ylmethyl)phenyl are used as functional monomers, and as sieving monomer it is used (2,4,5,5',4',4',5'-hexane(thiophene-2-yl)-3,3'-bitiophene. This printing is characterised by the use of a six-nucleotide sequence of single DNA or a peptide analogue of single DNA, i.e. PNA! In addition, the invention includes the application of the MIP recognition layer obtained by the invention,as part of a chemical sensor for the detection and/or selective determination of a six-nucleotide DNA sequence in the patient's bodily fluids using inhibitory capacity measurements (CI) under flow-through analysis (FIA). In addition, the invention includes the application of the MIP recognition layer to test the efficiency of the implementation and subsequent extraction of the TATAAA template and the attachment of the TATAAA analysis by the MIP layer by electrochemical impedance spectroscopy (EIS),a reflection of the infrared absorption spectroscopy (IRRAS) and differential pulse diameter (DPV) and the use of the MIP-recognition layer to test interactions with amino acids acting on the TATA tape using piezo-electric microgravity (PM) under FIA conditions. In particular, the invention applies to genetic engineering, in particular to genetic testing for early detection of hereditary genetic diseases and to basic research on the DNA transcription model.