A GENETICALLY ENGINEERED BACTERIAL STRAIN WSJ-IA I PRODUCING HIGH CONTENT AND HIGH YIELD OF ISOVALERYL SPIRAMYCIN

摘要

A GENETICALLY ENGINEERED STRAIN WSJ-IA FOR PRODUCING ISOVALERYL SPIRAMYCIN I. ALSO PROVIDED IS A METHOD FOR PREPARING THE STRAIN, COMPRISING THE STEPS OF: (A) CONSTRUCTING A RECOMBINANT PLASMID COMPRISING A DOUBLE GENE IST-ACYB2; (B) TRANSFORMING THE PLASMID INTO AN ISOVALERYL SPIRAMYCIN I - PRODUCING STRAIN TO OBTAIN THE STRAIN WSJ-IA. THE LEVEL OF ISOVALERYL SPIRAMYCIN I PRODUCED BY FERMENTATION OF THE STRAIN WSJ-IA IS INCREASED 1.7 TIMES AND THE FERMENTATION PROTENCY THEREOF INCREASED 4.14 TIMES IN COMPARISON WITH THE STRAIN EXCLUSIVELY COMPRISING A SINGLE GENE IST. FIG. 1