Device for electromechanical stimulation of muscular three-dimensional tissues or susceptible to being mechanically stimulated and cultured in vitro. (Machine-translation by Google Translate, not legally binding)

摘要

Device for electromechanical stimulation of muscular three-dimensional tissues or susceptible to being mechanically stimulated and cultivated in vitro, characterized essentially by a monobloc element preferably of silicone, in which a toroidal base of square or rectangular section and a thin membrane covering its central space, delimiting a culture space in the upper area of the toroidal base where the construct is deposited and a pneumatic chamber that occupies the lower part, being that the face of the membrane oriented to the culture space includes means for the mechanical fixation of the constructs constituted by protruding elements of the same and distributed in a homogeneous manner by its surface such as small rods, cubes, spheres, vertical membranes, or analogous elements at different scales. The construct deposited on the membrane is seated in the space between the fixation means, so that they pass through the three-dimensional fabric with what is mechanically fixed to the membrane. This surface can be functionalized or activated biochemically to increase the adhesion of the construct. The pneumatic chamber incorporates a gas passage through which periods of overpressure or depression are induced, which deform by extending the membrane. (Machine-translation by Google Translate, not legally binding)