multiresistant Staphylococcus immuno-detection and diagnostic kit

摘要

A method for detecting a bacterium that produces protein penicillin binding 2 ', comprising detection synthesizing enzyme cell wall binding protein penicillin 2' by immuno-based detection reaction antigen-antibody, which involves using a device immunochromatographic detection comprising on a support in sheet-like solid phase: a site supply of sample to a sample solution deduced to contain producing bacteria synthesizing enzyme cell wall binding protein is provided penicillin 2 'or a sample solution deduced protein containing a penicillin binding 2' released from the cell wall via sample pretreatment; a site labeled reagent which maintains a reagent is a labeled antibody that specifically binds to binding protein penicillin 2 'so that the reagent to extend through the solid phase support and a site of capture reagent in which he has immobilized a capture reagent that is an antibody that binds specifically to a site that is different from the site to which the labeled antibody binds and capturing a complex of penicillin binding protein 2 'and labeled reagent; wherein the capture reagent site comprises a sulfobetaine-type surfactant selected from the group consisting of CHAPS, CHAPSO, sulfobetaine, myristyl (SB3-14) and dodecyldimethylammonium butanesulfonate (DDABS), the method comprising supplying the sample solution to the site sample supply.