Method and device for determining the ratio of chlorophyll content and a chromophore compound in a plant tissue without independently measure the content

摘要

Procedure for determining the proportion of chlorophyll content (14) and a chromophore compound (13) in a plant tissue (T), not being said compound chromophore (13) fluorescent in the fluorescence band (22) of chlorophyll (14 said process the following operations), comprising: - emission by a first transmitter (11) in the direction of said plant tissue (T), of an optical radiation, called the first excitation radiation (111), selected to partially absorbed by compound chromophore (13) and to induce a first fluorescence radiation (112) of chlorophyll (14), - detection, by a first detector (15), a portion of a first fluorescence radiation (112) that is outside the absorption spectrum (21) of chlorophyll (14), - emission, by a second emitter (12) in the direction of said plant tissue (T), of an optical radiation, called second excitation radiation (121), non-selected absorbed by the compound chromophore (13) and to induce a second fluorescence radiation (122) of chlorophyll (14), - detection, by a second detector (16), a part of the second fluorescence radiation (122) is in the absorption spectrum (21) of chlorophyll (14), and - Determination of said ratio from the ratio of said fluorescence radiations that were detected.