METHOD OF DETECTING BLOOD PLASMA AMYGDALIN AFTER ADMINISTRATION OF FUZHENG HUAYU(FZHY)

摘要

A method of detecting blood plasma amygdalin after administration of Fuzheng Huayu (FZHY) is disclosed. The method includes: (1) adding 2-5 mol/L phosphoric acid to plasma of a mammal administered FZHY and mixing the solution of plasma-phosphoric acid (1:2-3, v/v), applying the solution to a Waters Oasis HLB small column activated by methanol and water; after leaching by water and 80-100% methanol and eluting by 0.2-1% ammonia-methanol, drying and enriching the eluent at 25-30 °C; and redissolving with mobile phase; (2) UPLC/MS measuring wherein the UPLC condition comprises an Acquity UPLC BEH C18, 2.1 x 100 mm chromatographic column, mobile phase A comprising water-acetonitrile-formic acid at a ratio (v/v/v) of 95:5:0.1, and mobile phase B comprising acetonitrile-formic acid at a ratio (v/v) of 100:0.1; and the MS condition comprises an electric spraying ion source (ESI), detecting with positive ion mode and scanning at the range of m/z 150-800. The method can be used for pharmacokinetics studies of amygdalin after administration of FZHY.