METHOD FOR MINUTELY REGULATING GENE EXPRESSION USING SYNTHESIS-REGULATING SRNA

摘要

The present invention relates to a method for minutely regulating gene expression using a synthesis-regulating sRNA in a prokaryotic cell and, more specifically, to a synthesis-regulating sRNA capable of minutely regulating the expression of a target gene by regulating the level of sRNA expression or the binding force between the sRNA and Hfq so as to minutely regulate the expression of the target gene. The method for minutely regulating gene expression using a synthesis-regulating sRNA, according to the present invention, can minutely regulate the degree of inhibiting the expression of a target gene by regulating the level of sRNA expression or the binding force between the sRNA and Hfq and thus can simultaneously, easily, and quickly apply various target gene combinations to various strains without gene deletion through the synthesis-regulating sRNA for regulating gene expression and is therefore very suitable for measuring the metabolizability of each strain and selecting an optimum strain. In addition, the method for minutely regulating gene expression using a synthesis-regulating sRNA has the advantages of easily and quickly selecting a target gene for the inhibition of gene expression and expressing the gene thus selected to a desired degree and thus can be used in producing recombinant strains for the efficient production of various metabolites and establishing a method for the efficient production and is therefore very useful.