ALL 'MINI-STR' MULTIPLEX WITH INCREASED C.E. THROUGH -PUT BY STR PROLONGATION TEMPLATE FUSION

摘要

A longstanding challenge in forensics is maximizing the number of "mini" - short tandem repeat amplicons within the limited confines of fluorescent channels and run length afforded by capillary electrophoresis. The instant disclosure overcomes this longstanding challenge by fusing mini - STR amplicons in a locus specific manner with a DNA construct of defined length. Staggering the lengths of the DNA constructs allows for staggering the mini - STRs in a locus specific manner. Beyond maximizing mini - STRs, the disclosed methods, compositions and kits can dramatically increase throughput of forensic samples by capillary electrophoresis.