COMMON HIGH-SPEED PHOTO-CROSS-LINKING LINKER FOR MOLECULAR INTERACTION ANALYSIS AND IN VITRO SELECTION, AND IN VITRO SELECTION METHOD USING LINKER

摘要

The purpose of the present invention is to provide a linker that can be used for both candidate clone screening and assessment of the binding of the resulting candidate clones, without using enzymes, and to provide an in vitro selection method using the linker. The present invention provides a common high-speed photo-cross-linking linker for molecular interaction analysis and in vitro selection comprising a main chain and a side chain. The main chain has: a solid-phase binding site located at the 5' terminus for forming a bond with a solid-phase; solid-phase cleavage site for separating the solid-phase by separating the entire solid-phase binding site; a side chain linking site for linking a side chain; a high-speed photo-cross-linking site for linking the main chain to mRNA having a sequence complementary to the main chain by photo-cross-linking; and a reverse transcription initiation region located adjacent to the side chain linking site at the 3' terminus of the main chain. The side chain has: a fluorescent label; a protein binding site located at the free terminus; and a link forming site that links to the side chain linking site on the main chain.