The present invention provides compositions and methods for recombinantly producing soluble and active microbial transglutaminases (m TGases). More particularly, the present invention relates to engineered microbial transglutaminase polypeptides comprising one or more substitutions in the pro-domain region to modulate the interaction between the pro-domain and the enzyme domain of the m TGase, and the nucleic acids encoding them. The present invention further relates to vectors and host cells comprising the engineered nucleic acids and polypeptides. The application further discloses methods of using microbial transglutaminases produced according to the methods of the disclosure.
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