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DNA RM18166 RM18171 Indel 15040 rh1 Composition for selecting green rice leafhopper-resistant variety containing Grh1 gene comprising DNA marker RM18166 RM18171 and Indel 15040
DNA RM18166 RM18171 Indel 15040 rh1 Composition for selecting green rice leafhopper-resistant variety containing Grh1 gene comprising DNA marker RM18166 RM18171 and Indel 15040
The present invention relates to a composition for selecting endemic hermit crab resistant rice varieties, which comprises a primer for detecting the presence or absence of one or more oligonucleotides or fragments thereof selected from the group consisting of RM18166, RM18171 and Indel 15040 as DNA markers, Comprising at least one primer set selected from the group consisting of SEQ ID NO: 1 to SEQ ID NO: 6. The present invention also relates to a method for isolating genomic DNA from a rice sample, Amplifying the target sequence by using the separated genomic DNA as a template and performing amplification reaction using at least one primer set selected from the group consisting of SEQ ID NO: 1 to SEQ ID NO: 6; And detecting the amplification product. The present invention also relates to a rice selection kit and a rice selection kit. According to the present invention, it is possible to efficiently distinguish endomycetes resistance and susceptibility by using a marker closely related to the position of the endomycetes resistance gene.
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机译:本发明涉及用于选择地方性寄居蟹抗性水稻品种的组合物,其包含用于检测一种或多种选自RM18166,RM18171和Indel 15040的寡核苷酸或其片段作为DNA标记的引物,包含选自由SEQ ID NO:1至SEQ ID NO:6组成的组的至少一个引物组。本发明还涉及一种从水稻样品中分离基因组DNA的方法,其通过使用分离的基因组来扩增靶序列。以DNA为模板,并使用选自由SEQ ID NO:1至SEQ ID NO:6组成的组中的至少一种引物组进行扩增反应。并检测扩增产物。本发明还涉及米选择套件和米选择套件。根据本发明,通过使用与内生菌抗性基因的位置紧密相关的标记,可以有效地区分内生菌抗性和易感性。
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