USE OF METHOD FOR THE DETECTION OF MULTIPLE TARGET NUCLEIC ACIDS USING CLAMPING PROBES AND DETECTION PROBES

摘要

The present invention relates to application of a target nucleic acid detection method using a clamping probe and a detection probe. According to the present invention, wild-type genes or amplification of undesired genes is prevented; an ultra-small amount of a target gene to be detected can be selectively amplified and detected; and consequently a small amount of mutation or a certain gene sequence included in a sample can be effectively detected. In addition, a large amount of gene types can be simultaneously detected by melting curve analysis. In particular, the target nucleic acid detection method can be used as a method for assisting medical diagnosis and prognosis of diseases, monitoring diseases, evaluating a treatment effect, and researching delivery of nucleic acid and protein by using an ultra-small amount of mutant genotype which can be detected at high detection sensitivity. The target nucleic acid detection method includes a step of detecting biomarkers such as EGFR, KRAS, NRAS and so on and determining whether a mutant of the biomarker exists by using a sample such as tissue, obtained by an invasive method, but also a sample such as blood, urine, sputum, excrement, saliva, and cells obtained by a non-invasive method. The biomarker and the mutant are used to monitor all periods of related diseases, prognose diseases, predict diseases, determine a treatment for diseases, diagnose diseases or diagnose disease early, prevent diseases, and develop a treating agent for diseases.