CELL-FREE PROTEIN SYNTHESIS METHOD AND DEVICE USING A EUKARYOTIC CELL LYSATE IN THE PRESENCE OF A CASPASE INHIBITOR AND THE USE OF A CASPASE INHIBITOR FOR INCREASING THE YIELD AND/OR THE STABILITY OF THE SYNTHESIZED PROTEINS IN SUCH A METHOD

摘要

The present invention relates to an improved method for cell-free protein synthesis using a eukaryotic cell lysate in the presence of a caspase inhibitor, an apparatus for carrying out the method and an increase in protein yield in a cell-free method for protein synthesis using a eukaryotic cell lysate And / or the use of a caspase inhibitor for increasing the stability of the synthesized protein. In a representative embodiment of the present invention, the method comprises at least two compartments separated by a dialysis membrane, wherein at least a first reaction chamber of the two compartments is subject to a translational reaction and during the translation reaction i) From at least the other feed and discharge compartments into the reaction compartment through the dialysis membrane and ii) the reaction by-products are dispersed from the reaction compartments into the feed and discharge compartments through the dialysis membrane. According to the present invention, the caspase inhibitor may also be present in the feed and discharge compartments to supply additional fresh inhibitors, but is certainly present in at least the reaction compartment.