CONDITIONED CULTURE MEDIUM CULTIVATED WITH ADIPOSE-DERIVED STEM CELLS HAVING IMPROVED HAIR GROWTH AND HAIR LOSS PREVENTION ACTIVITY AND METHOD FOR PREPARING THE SAME

摘要

Provided is a manufacturing method of a serum-free cell culture fluid, which comprises: an adipose stem cell culturing step for culturing adipose stem cells in a serum-free medium; a co-culturing step for co-culturing the cultured adipose stem cells and monocyte cells in the serum-free medium for 70 to 150 hours; and removing cells and floating matter from the co-cultured cell culture fluid, and obtaining only the cultured product. The culture fluid of adipose stem cells with improved hair growth and hair loss preventive activities minimizes side effects such as immune rejection response or the like.