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METHOD FOR PRODUCING POLYCLONAL ANTIBODIES USING AN ANTIGENIC COMPOSITION COMPRISING PROTEIN-CONTAINING MEMBRANE VESICLES
METHOD FOR PRODUCING POLYCLONAL ANTIBODIES USING AN ANTIGENIC COMPOSITION COMPRISING PROTEIN-CONTAINING MEMBRANE VESICLES
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机译:使用包含蛋白质的膜囊泡的抗原组合物制备多克隆抗体的方法
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摘要
The present invention provides a method of producing a polyclonal antibody corresponding to an antigenic target protein, the method comprising: applying an immunogenic composition comprising a membrane vesicle comprising the antigenic target protein to a host to induce an immune response in the host; And obtaining an antibody corresponding to the target protein in the serum of the host. More specific embodiments of the method include at least the following steps: a) synthesis of the target protein via in vitro translation method in a reaction solution comprising a cell lysate containing a nucleic acid template and a membrane vesicle coating the target protein step; b) separating membrane vesicles containing the target protein synthesized in solution; c) introducing a membrane vesicle containing the synthesized target protein into a physiologically compatible solution; d) applying the membrane vesicles of step c) to the host; e) testing a specific immune response to the target protein with a serum sample of the host; And f) obtaining a specific antibody corresponding to the target protein from the serum of the host showing a specific translational response to the target protein.
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