CODON-OPTIMIZED PROTEORHODOPSIN FOR USING PHOTIC DRIVING ENERGY METABOLIC PATHWAY IN NON-PHOTOSYNTHETIC MICROORGANISMS, AND USE OF SAME

摘要

The present invention relates to a codon-optimized proteorhodopsin gene for using a photic driving energy metabolic pathway in non-photosynthetic microorganisms. Moreover, the present invention relates to an expression vector including the gene; a microorganism transformed by the expression vector; and a method for producing proteorhodopsin by using the microorganism. Furthermore, the present invention relates to a method for culturing the microorganism for using a photic driving energy metabolic pathway. A SAR86_Opt gene synthesized through codon optimization according to the present invention can effectively induce proteorhodopsin expression in a host cell which is a non-photosynthetic microorganism under a photic irradiation condition. In addition, inducing the expression of proteorhodopsin causes maintenance of metabolic pathway energy needed in growing cells. An induced photic driving energy metabolic pathway is used for metabolic energy of a host cell, inducing extinction or late arrival of a death phase according to extension of a stationary phase. Also, an increase in a production amount of metabolites and an increase in a production amount of target proteins can be induced in E. coli. Accordingly, by using the present invention as an industrial microorganism cell, it is expected that sustainable precursor materials are produced and yields are improved.;COPYRIGHT KIPO 2016