The present invention relates to a novel iron-dependent GH16 -agarase AgaH92 derived from Pseudoalteromonas sp. H9, and specifically, to -agarase which is identified from a strain of Pseudoalteromonas sp. H9, allows overexpression in a hetero strain, has thermal stability, and recognizes -combination of agar so neoagaro-oligosaccharide can be generated. According to the present invention, the -agarase is greatly effective for decomposing agar into small molecules, can be massively produced by an overexpression system using a hetero host cell, and is highly stable even against heat. Therefore, the -agarase can be highly practically used with different enzymes in the field of industry to produce various compounds or energy by decomposing or converting agar. Furthermore, in the case of using the -agarase in the present invention, effective neoagaro-oligosaccharides such as neoagarotetraose or neoagarohexaose can be efficiently produced from agar.
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