Single point nucleotide changed Live Attenuated Viral Hemorrhagic Septicemia Virus

摘要

In a viral hemorrhagic septicemia virus (VHSV) virus, the present invention relates to a method for detecting the presence of a pan-handle RNA secondary structure at the 3'-5 'untranslated region end of the nucleoside sequence Provides host-independent attenuated viral hemorrhagic sepsis virus that converts guanine or uracil to the 4 ' -terminal adenine at the internal loop opening point. The attenuated vaccine prepared by the method of the present invention is advantageous in that the attenuated VHSV is produced through minimal anthropogenic change and the stability is remarkably improved as compared with the existing attenuated vaccine.