首页> 外国专利> Application of the new isolates PSEUDOMONAS SP. IPB-A36 from an environment for efficient production of medium / long-chain-PGA and PGA SPECIALIZED

Application of the new isolates PSEUDOMONAS SP. IPB-A36 from an environment for efficient production of medium / long-chain-PGA and PGA SPECIALIZED

机译:新分离株PSEUDOMONAS SP的应用。 IPB-A36来自有效生产中/长链-PGA和PGA的环境

摘要

1. The microorganism of the genus Pseudomonas, deposited at DSMZ as a DSM26198.2. A method of producing a medium- or long-chain PHAs vklyuchayuschiykultivirovanie microorganism of the genus Pseudomonas, deposited at DSMZ like DSM26198, in a culture medium containing a carbon source u isolation from microorganism PGA.3. A method according to claim. 2, where the nutrient medium is CY medium, preferably CY medium (2N) .4. A method according to claim 2 or 3, wherein the carbon source soderzhit- at least one C4-C20 fatty acid, preferably C8-C18 fatty acid, said fatty acid (s) optionally contains one or more unsaturated groups, -., At least one carboxylic acid containing an aromatic group, preferably phenyl-substituted ω-fatty acid, more preferably having a fatty acid chain of 4-10 carbon atoms, or smesi.5. A method according to claim. 4, wherein the mixture of saturated and / or unsaturated fatty acids and carboxylic acids containing one or more unsaturated functional groups together is fed to the culture sredu.6. A method according to any one of claims. 2 or 3, wherein nitrogen is present in the culture medium in the form of ammonium salts, preferably ammonium molar concentration in the range of 8 to 30 mmol, in particular in the range from 10 to 20 mmol.7. A method according to any one of claims. 2 or 3, wherein the method is carried out in shake flasks or batchwise, and the ratio of carbon to nitrogen (C / N) in the culture medium is in the range of about 20 to 45, preferably from about 25 to 35.8. A method according to any one of claims. 2 or 3 wherein the culture medium is supplied by feeding a carbon source for the growing of exponent
机译:1.假单胞菌属微生物,以DSM26198.2。的形式存放在DSMZ中。一种在含有碳源的培养基中生产假单胞菌属的中链或长链PHA假单胞菌属微生物的方法,该培养基如DSM26198一样沉积在DSMZ上。3。根据权利要求1的方法。 2,其中营养培养基是CY培养基,优选CY培养基(2N).4。 4.根据权利要求2或3所述的方法,其中所述碳源为至少一种C 4 -C 20脂肪酸,优选为C 8 -C 18脂肪酸,所述脂肪酸任选地包含一个或多个不饱和基团。一种包含芳族基团的羧酸,优选为苯基取代的ω-脂肪酸,更优选具有4-10个碳原子的脂肪酸链,或1.5。根据权利要求1的方法。参照图4,其中将饱和和/或不饱和脂肪酸与一起包含一个或多个不饱和官能团的羧酸的混合物进料到培养液中6。根据权利要求1的方法。参见图2或3,其中氮以铵盐形式存在于培养基中,优选铵的摩尔浓度为8至30mmol,特别是10至20mmol.7。根据权利要求1的方法。参照图2或3,其中该方法在摇瓶中或分批进行,并且培养基中碳与氮的比率(C / N)为约20至45,优选约25至35.8。根据权利要求1的方法。参照图2或3,其中通过供给碳源以供指数生长来提供培养基

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