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Application of the new isolates PSEUDOMONAS SP. IPB-A36 from an environment for efficient production of medium / long-chain-PGA and PGA SPECIALIZED
Application of the new isolates PSEUDOMONAS SP. IPB-A36 from an environment for efficient production of medium / long-chain-PGA and PGA SPECIALIZED
1. The microorganism of the genus Pseudomonas, deposited at DSMZ as a DSM26198.2. A method of producing a medium- or long-chain PHAs vklyuchayuschiykultivirovanie microorganism of the genus Pseudomonas, deposited at DSMZ like DSM26198, in a culture medium containing a carbon source u isolation from microorganism PGA.3. A method according to claim. 2, where the nutrient medium is CY medium, preferably CY medium (2N) .4. A method according to claim 2 or 3, wherein the carbon source soderzhit- at least one C4-C20 fatty acid, preferably C8-C18 fatty acid, said fatty acid (s) optionally contains one or more unsaturated groups, -., At least one carboxylic acid containing an aromatic group, preferably phenyl-substituted ω-fatty acid, more preferably having a fatty acid chain of 4-10 carbon atoms, or smesi.5. A method according to claim. 4, wherein the mixture of saturated and / or unsaturated fatty acids and carboxylic acids containing one or more unsaturated functional groups together is fed to the culture sredu.6. A method according to any one of claims. 2 or 3, wherein nitrogen is present in the culture medium in the form of ammonium salts, preferably ammonium molar concentration in the range of 8 to 30 mmol, in particular in the range from 10 to 20 mmol.7. A method according to any one of claims. 2 or 3, wherein the method is carried out in shake flasks or batchwise, and the ratio of carbon to nitrogen (C / N) in the culture medium is in the range of about 20 to 45, preferably from about 25 to 35.8. A method according to any one of claims. 2 or 3 wherein the culture medium is supplied by feeding a carbon source for the growing of exponent
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