SEQUENCES FOR DETECTION AND RECOGNITION OF METICILLIN-RESISTANT GOLDEN STAFILOCOCCUS (STAPHYLOCOCCUS AUREUS) (MRSA) WITH MREJ TYPE XXI

摘要

1. Composition for detecting methicillin-resistant Staphylococcus aureus (MRSA) containing MREJ type xxi nucleic acids, said S. aureus contains the staphylococcal chromosomal cassette element mec (SCCmec), including the mecA homologue (mecA), said SCCmec cassette is integrated into S chromosomal DNA . aureus, thereby forming a polymorphic sequence of type xxi region of the connection at the right end (MREJ), which includes polymorphic sequences at the right end and chromosomal DNA adjacent to the indicated polymorphic sequences at the right end, the specified comp The position contains: a first amplification primer, said first amplification primer has a length of from 10 to 45 nucleotides, wherein said first amplification primer specifically hybridizes under standard PCR conditions with polymorphic sequences at the right end of MREJ type xxi nucleic acids. 2. The composition of claim 1, wherein said first amplification primer specifically hybridizes to the nucleic acid sequence of SEQ ID NO: 1 or to a sequence complementary to it under the indicated standard PCR conditions. 3. The composition of claim 1, further comprising: a second amplification primer, said second amplification primer having a length of from 10 to 45 nucleotides, wherein said second amplification primer specifically hybridizes under standard PCR conditions with S. aureus chromosomal sequences located in within 1 kb from the point of insertion of the SCCmec element into the chromosomal DNA, and the indicated first and second primers for amplification together form an amplicon of the region of the compound at the right end of MREJ type xxi nucleic acids under standard PCR conditions in the case of