Recombinant plasmid DNA pET22b (+) / slurp-2 encoding the SLURP-2 protein and the bacterial strain Eschrichia coli BL21 (DE3) pET22b (+) / slurp-2-producer of human SLURP-2 protein

摘要

1. Recombinant plasmid DNA pET22b (+) / Slarp-2 encoding a protein of human Slarp-2 5616 bp, consisting of NdeI / HindIII - DNA fragment of plasmid pET22b (+) 5382 bp long, containing the lac promoter E. coli, β-lactamase bla gene, E. coli lacI gene fragment, replication initiation ori region and artificial DNA sequence encoding a 240 bp NdeI / HindIII fragment including the synthetic human Slarp-2 gene; containing unique recognition sites by restriction endonucleases having the following coordinates: XhoI - 158, HindIII - 173, EcoRI - 192, BamHI - 198, NdeI - 430, BglII - 534, MluI - 1256, PstI - 4495.2. The bacterial strain Escherichia coli BL21 (DE3) / pET22b (+) / Slarp-2 is a producer of human Slarp-2 protein.