METHOD FOR DETERMINING THE TOTAL ACTIVITY OF ANTIMICROBE PEPTIDES AS A MARKER OF THE STATE OF LOCAL IMMUNITY OF VARIOUS EPITELIAL TISSUES

摘要

1. A method for determining the total activity of antimicrobial peptides as a marker of the state of local immunity of various epithelial tissues, which consists in collecting as a sample the secretion of epithelial tissue in the form of saliva or urine or vaginal secretion or collecting a water-soluble fraction of skin secretion, then a sample exempt from epithelial cells and bacteria by filtration and / or centrifugation, and then an aliquot of this sample is combined in a certain ratio with a previously prepared suspension of exponential cells of Candida albicans of a certain optical density, characterized in that the mixture prepared by the above method is incubated in a thermostat for 2 hours, centrifuged, and a solution of bromocresol purple is added to stain the killed cells, then thermostatted and centrifuged again, and the resulting precipitate is microscopically photographed on a digital camera and, after transferring the resulting digital image to a computer, dead cells are counted, the percentage of which reflects the total AMP activity. 2. The method according to claim 1, characterized in that the precipitate obtained is microscopic with a total magnification of the microscope of at least 1750x.3. The method according to claim 1 or 2, characterized in that the selection of the ratios between the volumes of the epithelial secretion sample and the suspension of Candida albicans yeast cells is carried out empirically so that the maximum activity for samples of this type does not exceed 95%.