REGULATORY NUCLEIC ACID MOLECULES FOR STRENGTHENING SEED-SPECIFIC AND / OR SEED-PREFERRED GENE EXPRESSION IN PLANTS

摘要

1. A method of obtaining a seed-specific plant expression promoter of high expression, containing functional binding to the promoter of one nucleic acid molecule that enhances the expression of nucleic acid (NEENA), heterologous with respect to the specified promoter, selected from i) a nucleic acid molecule having the sequence as defined in SEQ ID NO: 6-15, wherein expression originating from said seed-specific promoter functionally linked to said NEENA molecule is increased compared to the same with ER-specific promoter is not operably linked to said NEENA.2 molecule. The method of claim 1, comprising the steps of a) introducing one NEENA, a selected nucleic acid molecule of claim 1 i), into a plant or part thereof, and b) integrating said NEENA into the genome of said plant or part thereof, whereby said one NEENA is operably linked with an endogenous seed-specific expressed nucleic acid heterologous with respect to said NEENA alone and, if necessary, c) regenerating a plant or a part thereof containing said NEENA alone from said transformed cell. 3. The method of claim 1, comprising the steps of a) providing an expression construct containing one NEENA of the selected nucleic acid molecule of claim 1 i) operably linked to a seed-specific and promoter and to one or more nucleic acid molecules, the latter being heterologous in with respect to the specified one NEENA, and which is under the control of the specified seed-specific promoter, and b) integration of the specified expression constructs containing the specified one NEENA, in