METHOD OF GENERATING CYTOTOXIC CELLS WITH ACTIVITY AGAINST NON-SMALL CELL LUNG CANCER CELLS

摘要

FIELD: biotechnologies.;SUBSTANCE: invention relates to biotechnology, particularly to immunology, and can be used for generation of cytotoxic cells with activity against non-small cell lung cancer. Method involves combined cultivation in presence of recombinant human interleukine-12 and recombinant human interleukin-18, non-adhesive fraction of mononuclear cells (MNC) recovered from peripheral blood of patient with non-small cell lung cancer with dendritic cells obtained from monocytes of adhesive MNC fraction. To produce mature DC after three days of cultivation of adhesive MNC fraction, obtained immature DC are first co-cultivated for one day with tumour cell lysate non-small cell lung cancer with subsequent addition of TNF-α. During one day after loading lysate simultaneously with addition of proinflammatory cytokine TNF-α cytokine IL-1 is addedβ and matured loaded with lysate in presence of both cytokines.;EFFECT: higher efficiency of generation of cytotoxic cells with activity against non-small cell lung cancer.;1 cl, 1 tbl