RECOMBINANT BACTERIAL STRAIN Escherichia coli N42 (pElmI) - PRODUCER OF METHYL-DEPENDANT SITE-SPECIFIC ENDONUCLEASE ElmI

摘要

FIELD: biotechnology.;SUBSTANCE: invention represents recombinant bacterial strain Escherichia coli N42 (pElmI) being a producer of methyl-dependant site-specific DNA-endonuclease ElmI splitting both chains of DNA sequence 5′-GC^NGC-3′/3′-CGN^CG-5′, in which at least three cytosines are methylated in the C5 position to form 5′-projecting nucleotide ends. Strain is produced by cells transformation of Escherichia coli ER2267 with pElmI plasmid. This plasmid is designed on the basis of vector pMTL22 and contains a gene coding the methyl-dependant site-specific DNA-endonuclease ElmI from the natural strain Escherichia coli N17.;EFFECT: invention enables to obtain DNA-endonucleases ElmI of a specified specificity with higher output.;1 cl, 4 dwg, 4 ex