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Magnetic flow cytometry to the individual cell detection
Magnetic flow cytometry to the individual cell detection
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机译:电磁流式细胞仪检测单个细胞
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摘要
A process for the magnetic flow measurement of cells (10), wherein said method comprising the steps of:– Putting of a sensor arrangement, wherein in order to start up at least a first (r1, r2, r3) and a second magnetoresistive component (r2, r3, r4) in a - of the wheatstone bridge in the diagonal and crossing arrangement (40) or a parallel arrangement (90) and connected at a distance (δx) from one another in the direction of flow (20) are arranged,– magnetic marking of the cells (10),– Flow generation of the cells by means of the sensor arrangement, wherein the flow (20) of the cells (10), first via the first (r1, r2, r3) and then over the second magnetoresistive component (r2, r3, r4) is guided,– Individual cell detection, wherein by the magnetic field of an individual magnetically labelled cell (10), which flows via the sensor arrangement, a test signal with a characteristic measuring signal pattern of at least three measuring deflections is generated– Evaluation of the measuring signal, in the deflection on the basis of the measuring direction of the sequence of the measuring signal is identified as an individual cell detection;characterized in that– from the characteristic measuring signal pattern is a mess deflection distance (δt) is evaluated and the cell diameter on the basis of the measuring deflection distance (δt) is calculated.
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