Method for the determination and evaluation of time-resolved fluorescence or reflection images on extended three-dimensional surfaces

摘要

The present invention relates to a method for determining and evaluating time-resolved fluorescence or reflection images on extended three-dimensional surfaces which are scanned by pulsed radiation (pointwise optical scanning), for example for tissue characterization and diagnosis in medicine or for examination on mechanically difficult to access technical objects (such as in an explosion-proof environment). The object of the invention to provide a method for determining and evaluating time-resolved fluorescence or reflection images on extended three-dimensional surfaces is achieved in that for the determination and evaluation of parameters of time-resolved fluorescence or reflection images on a three-dimensional surface (230) of objects in which a pointwise pulsed scanning of the three-dimensional surface (230) by means of illumination and detection beam, wherein different distances of the illumination beam exit and detection beam entry point (210) from the pulsed illuminated points of the three-dimensional object (230) are present and a lit first object point (231) and a lit. second object point (232), which is located at a different distance to the illumination beam exit and detection beam entry point (210), in the duration of the excitation and detection beam (221) of the first Obj ectpunkts (231) and the illumination and detection beam (222) of the second object point (232) and a pixel-by-pixel modeling of the different maturities takes place, with different maturities in the determination of the fluorescence lifetime from the different distances of the illumination beam exit point or Detection beam entry point and between different illuminated surface points result, to be considered in a model function.