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Apparatus and method for the detection and quantification of single-stranded target - nucleic acids

机译:用于检测和定量单链靶标-核酸的装置和方法

摘要

The invention relates to a device and a method for the detection and quantification of single-stranded target - nucleic acids. The device comprises at least one solid support, on the at least a double-stranded catcher - oligonucleotide and at least a double-stranded reporter - oligonucleotide are immobilized. The catcher - oligonucleotide comprises at least one first oligonucleotide - sequence, which leads to a target - nucleic acids partially complementarily, and the reporter - oligonucleotide comprises at least a second oligonucleotide - sequence that, to the target - nucleic acids is partially complementarily. Furthermore, the apparatus comprises a read unit for reading out a mark of the reporter - oligonucleotide on the support.
机译:本发明涉及用于检测和定量单链靶核酸的装置和方法。该装置包括至少一个固相支持物,该至少一个固相支持物被固定在至少一个双链捕集体-寡核苷酸和至少一个双链报道分子-寡核苷酸上。捕获物-寡核苷酸包含至少一个第一寡核苷酸-序列,其导致靶标-核酸部分互补,而报道分子-寡核苷酸包含至少第二寡核苷酸-序列,其与靶标-核酸部分互补。此外,该设备包括用于读取报告基团上的报告子-寡核苷酸的标记的读取单元。

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