Apparatus for producing a skin protein of esox lucius

摘要

Apparatus for producing a skin proteins, preferably of esox lucius, comprising:a squeeze-off - and cleaning device (10) for thawing a frozen skin sample, preferably carp skin or hecht skin (esox lucius), for 12 hours at 7°C, and in which the thawed skin, preferably the carp skin, then with cleaning water at a temperature of about l0°C. can be cleaned,a skin slags, the processor (15), which is connected with the squeeze-off - and cleaning device (10) is connected and for the preparation of a degreased skin slag is used, wherein in the skin slags, the processor (15) which, in the upstream squeeze-off - and cleaning device (10) purified skin with a beater assembly to a skin mass mashed, and to this skin mass then is added to water, wherein the skin mass with the water in the case of the constriction in a colloid mill (16) is milled to a skin slag, wherein the skin slag is centrifuged, wherein the upper layer of fat of the centrifuged skin slag is removed in order to obtain the degreased skin slag,an ultrasonic generator (20), which is connected to the skin slags, the processor (15) is connected to receive the defatted skin slag is used, wherein the organizational structure of the skin proteins in the slag by means of an ultrasound treatment in the case of a power of 800 w and a frequency of 25 khz for 10 minutes is changed,a composite dilute solutions ezu sensor (25), which is connected to the ultrasonic generator (20) is connected, wherein the composite protease, to the ultrasonic generator (20) processed slag is added, wherein the composite protease a mixture of an alkaline protease and a neutral protease is, which, in a mass ratio of 1: 1 are mixed, wherein the slag, after addition of the composite protease at a temperature of 50°C. and a ph - value of 7,5 for 0,5 hours can be digested,a collector (30), the dilute solutions with the composite element transducer (25) is connected, and for receiving the slag from the composite dilute solutions ezu sensor (25), and in which the slag at a temperature of 95°C for a certain time remains and is then cooled to ambient temperature, wherein for 10 minutes, a centrifugation at 4000g takes place, wherein then a projection on the surface of the centrifuged slag is removed, whereby the molecular weight of the skin protein in the supernatant between 6000 – 8000 daltons is,a two-stage filter (35), of the connected to the collector (30) is connected to the supernatant from the collector (30) for a two-stage ultrafiltration process is to be received, wherein the projection in a first step with a ceramic ultrafilter (36) having a pore size of 0,5 μm is filtered, wherein in the case of the filtration with the ceramic ultrafilter (36) the proteins having a molecular weight of less than 15000 of the poly peptide mixture in the supernatant is separated. accordingly, further comprising a ceramic filter (37) having a pore size of 2 μm is employed, in order that the proteins having a molecular weight of 6000 – 8000 daltons to a skin protein having a molecular weight of 6000 – 8000 daltons to obtain.