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NOVEL ANTIBODY PURIFICATION METHOD AND ANTIBODY OBTAINED THEREFROM, AND NOVEL ANTIBODY PURIFICATION METHOD USING CATION EXCHANGER AND ANTIBODY OBTAINED THEREFROM
NOVEL ANTIBODY PURIFICATION METHOD AND ANTIBODY OBTAINED THEREFROM, AND NOVEL ANTIBODY PURIFICATION METHOD USING CATION EXCHANGER AND ANTIBODY OBTAINED THEREFROM
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机译:新型抗体纯化方法和获得抗体的方法,以及利用阳离子交换剂和抗体获得的新型抗体纯化方法
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摘要
The first embodiment of the present invention is a method for purifying an antibody or a substance derive from an antibody, wherein a carrier 1 having an affinity ligand with affinity for the antibody or the substance derived from the antibody and a carrier 2 having a cation exchange group are used to prepare an integrated column 1 connecting a column containing the carrier 1 and a column containing the carrier 2 or a column 2 having the mixture of the carrier 1 and carrier 2, the antibody or the substance derived from the antibody is applied to the column 1 or the column 2, and then the adsorbed antibody or substance derived from the antibody is eluted from the column 1 or the column 2. The second embodiment of the present invention is a method for using a carrier having a cation exchange group, wherein a solution containing an antibody or a substance derived from an antibody is applied to a carrier having a cation exchange group having a carboxyl group-containing ligand and pKa of 4.0 or more, and the antibody or the substance derived from the antibody is eluted by an acidic buffer having pH of 4.0 or less.
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