METHOD FOR DETECTING BACTERIA CAUSING BACTERIAL FRUIT BLOTCH DISEASE AND BACTERIA CAUSING BACTERIAL BROWN STRIPE DISEASE IN CUCURBITACEAE

摘要

PROBLEM TO BE SOLVED: To provide a method for detecting bacteria causing bacterial fruit blotch disease while distinguishing them from their closely related species, bacteria causing bacterial brown stripe disease, in Cucurbitaceae.SOLUTION: The method for detecting bacteria causing bacterial fruit blotch disease and bacteria causing bacterial brown stripe disease in Cucurbitaceae comprises:performing a nucleic acid amplification reaction on DNA obtained from a cucurbitaceous plant using (A) a primer consisting of a specific nucleotide sequence and a primer consisting of a nucleotide sequence complementary to nucleotides of the specific nucleotide sequence and (B) a DNA/RNA chimeric probe consisting of nucleotides of a specific sequence, where RNA is the only one base or consecutive 2 to 3 bases including the base corresponding to the specific sequence and base G or A corresponding to position 127 of the specific sequence is included.SELECTED DRAWING: None