A well-tolerated and highly specific tailor-made recombinase for recombination of asymmetric target sites in multiple retrovirus strains

摘要

The present invention is well tolerated, capable of recombining asymmetric target sequences within the long terminal repeat (LTR) of proviral DNA of multiple retroviral strains that can be inserted into the genome of a host cell. In addition to methods for preparing expression vectors encoding highly specific tailored recombinases, the resulting expression vectors, cells transfected with these expressed recombinases, and expression vectors, cells and / or recombinases It is related with the pharmaceutical composition containing. The pharmaceutical composition is useful, for example, in the treatment and / or prevention of retroviral infections, particularly HIV infection. In particular, the present invention combines well-tolerated and highly specific tailor-made recombinases capable of combining asymmetric target sequences in more than 90% of HIV strains, thereby excising HIV-1 sequences, and The present invention relates to an expression vector encoding