Generation of mesenchymal stromal cell banks from mononuclear cells of pooled multiple bone marrow donors

摘要

The present invention relates to improved mesenchymal stromal cell (MSC) preparations and methods of making the same. The present invention provides a new strategy for isolating MSCs from BM-MNC by pooling bone marrow mononuclear cells (BM-MNC) from multiple unrelated (third party) bone marrow donors. MSC preparations produced according to the methodology of the present invention are characterized by stable growth potential and increased immunosuppressive capacity compared to individual donor MSC preparations or pools of individual MSCs generated from multiple donors. To do. MSCs prepared according to the present invention are particularly useful as recipients of hematopoietic stem cell transplantation, for medical applications such as the treatment of graft-versus-host disease (GvHD) in patients with autoimmune disorders, and as a cell-based therapy in regenerative medicine. Useful. [Selection figure] None