NMR structure analysis method of stable isotope-labeled aliphatic amino acid and protein using it

摘要

Provided is a stable isotope-labeled aliphatic amino acid enabling the assignment of the signal of an amino acid residue side chain by increasing to the maximum the observation sensitivity to an NMR signal of the same amino acid residue side chain, and allowing NOE (nuclear Overhauser effect) between protons in the amino acid residue to be observed. The stable isotope-labeled aliphatic amino acid is for constituting a protein and satisfies all of the following conditions (1) to (3): (1) two or more carbon atoms are labeled with 13C; (2) of two or more carbon atoms labeled with 13C, a carbon atom other than a carbon atom of a methyl group, which is capable of bonding to a hydrogen atom, has one 1H directly bonded thereto, while the carbon atom of the methyl group has at least one 1H directly bonded thereto; and (3) other carbon atoms adjacent to all the 13C are all 12C.