Method for detecting fluorescence or absorbance, background suppression method, measurement method for ADP, activity measurement method for enzyme producing ADP, and method for measuring activity of glycosyltransferase

摘要

The method for detecting fluorescence or absorbance of the present invention is characterized in that resazurin is reduced to resorufin by diaphorase in the presence of the SH reagent and NADH or NADPH and the resulting fluorescence intensity or absorbance is measured. The method for measuring ADP of the present invention comprises a 2-1 step of allowing glucose to act on ADP and ADP-dependent hinase and a step 2-1 for adding glucose-6-phosphate obtained by said 2-1 step with NAD or NADP and A second 2-2 step of allowing glucose-6-phosphate dehydrogenase to act, and a step of reacting resazurin with NADH or NADPH and diaphorase obtained in said 2-2 step in the presence of SH reagent, And a second 2-3 step of measuring the absorbance.