首页>
外国专利>
Discovery and Validation of Cancer Biomarkers Using a Protein Analysis Methodology to Analyze Specimens
Discovery and Validation of Cancer Biomarkers Using a Protein Analysis Methodology to Analyze Specimens
展开▼
机译:使用蛋白质分析方法分析标本的癌症生物标志物的发现和验证
展开▼
页面导航
摘要
著录项
相似文献
摘要
Methods are provided for the analysis, including the serial analysis, of very small samples of tissue. The methods utilize a nanofluidic proteomic immunoassay (NIA) to quantify total and low-abundance protein isoforms in a small amount of lysate. NIA detection accurately measure oncoprotein expression and activation in limited clinical specimens, including isoforms that differ in post-translational modifications, such as phosphorylation, and the like. The NIA detection method combines isoelectric protein focusing and antibody detection in a nanofluidic system.
展开▼