INCREASING CELLULAR LIPID PRODUCTION BY INCREASING THE ACTIVITY OF DIACYLGLYCEROL ACYLTRANSFERASE AND DECREASING THE ACTIVITY OF TRIACYLGLYCEROL LIPASE

摘要

Disclosed are methods and compositions for increasing the triacylglycerol content of a cell by up-regulating diacylglycerol acyltransferase and down-regulating triacylglycerol lipase. In some embodiments, a DGA1 protein is expressed and a native TGL3 gene is knocked out, thereby increasing the synthesis of triacylglycerol and decreasing its consumption, respectively.