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FUNCTIONALIZED PARTICLES FOR LABEL-FREE DNA IMPEDIMETRIC BIOSENSOR FOR DNA AND RNA SENSING
FUNCTIONALIZED PARTICLES FOR LABEL-FREE DNA IMPEDIMETRIC BIOSENSOR FOR DNA AND RNA SENSING
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机译:用于DNA和RNA传感的无标签DNA阻抗生物传感器的功能化颗粒
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In one embodiment, 3-Aminopropyltriethoxysilane (APTES) functionalized graphene oxide (APTES-GO) wrapped SiO2 particle composite (SiO2@APTES-GO) was prepared via the self-assembly process of APTES-GO sheets and SiO2 particles. Transmission electron microscopy (TEM) and Attenuated Total Reflectance-Fourier Transform Infrared spectroscopy (ATR-FTIR) confirmed wrapping of the SiO2 particles by the APTES-GO sheets. A biosensor based on electrochemical impedance spectroscopy (EIS) was constructed and used to sensitively detect dengue DNA and dengue RNA via primer hybridization using different oligonucleotide sequences. The results demonstrated that the SiO2@APTES-GO electrode material led to enhanced sensitivity, selectivity and detection limit, compared to both APTES-GO and APTES-SiO2. The three-dimensional structure, high surface area, electrical properties and the ability for rapid hybridization offered by the SiO2@APTES-GO rendered this electrode material as ideal to use in the reported dengue impedimetric sensor.
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